4 Key Factors That Will Affect Your Pipetting Accuracy

Pipetting Accuracy

Pipette Calibration and Pipetting Accuracy

A pipette is a high precision laboratory instruments that requires proper maintenance on a regular basis. Not calibrating your pipette on a regular basis is like driving your car and never giving it an oil change; eventually you’re going to run into major problems with pipetting accuracy.

Pipette Storage – Where and How for Pipetting Accuracy

Proper storage of your pipette is a very important factor in maintaining an accurate pipetting result. Here are a few tips on where and how to store your pipettes:

  • Upright – Storing your pipette in an upright position will help prevent any liquids from corroding the internal workings of your pipette
  • Highest Volume – Mechanical pipettes use a spring loaded piston assembly to adjust the volume. Storing your pipette at the highest volume will reduce stress on the spring loaded assembly and reduce your risk of any issues with your pipettes
  • Environmental Factors – Store your pipettes away from the window, heating sources and in an environment with low moisture

Pipette Technique for Pipetting Accuracy

Maintaining the proper pipette position and choosing the right technique can improve the accuracy of your pipetting results.

Pipette position

Pipetting technique

Choosing the right pipetting technique is highly dependent upon on the consistency of the samples you are pipetting. For example, reverse pipetting is a better technique for pipetting more viscous liquids where as regular pipetting (also called forward pipetting) is better for more aqueous solutions.

Pipette Tips

Your pipette is not a complete system without the tip. Thus, choosing the right pipette tip is a very important factor in achieving accurate results.

Here are a few key rules to live by when choosing the right pipette tip:

All Tips should be:

  • Manufactured by the company that manufactured your pipette.
  • Made of high quality polypropylene
  • Free from impurities such as particles and dust
  • Highly resistant to chemicals
  • Stable for high temperatures
  • Free from scratches, air bubbles and bends